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- Title
Granular Layer Neurons Control Cerebellar Neurovascular Coupling Through an NMDA Receptor/NO-Dependent System.
- Authors
Gagliano, Giuseppe; Mapelli, Lisa; Soda, Teresa; D'Angelo, Egidio U.; Laforenza, Umberto; Moccia, Francesco
- Abstract
Neurovascular coupling (NVC) is the process whereby neuronal activity controls blood vessel diameter. In the cerebellum, the molecular layer is regarded as the main NVC determinant. However, the granular layer is a region with variable metabolic demand caused by large activity fluctuations that shows a prominent expression of NMDA receptors (NMDARs) and nitric oxide synthase (NOS) and is therefore much more suitable for effective NVC. Here, we show, in the granular layer of acute rat cerebellar slices, that capillary diameter changes rapidly after mossy fiber stimulation. Vasodilation required neuronal NMDARs and NOS stimulation and subsequent guanylyl cyclase activation that probably occurred in pericytes. Vasoconstriction required metabotropic glutamate receptors and CYP ω-hydroxylase, the enzyme regulating 20-hydroxyeicosatetraenoic acid production. Therefore, granular layer capillaries are controlled by the balance between vasodilating and vasoconstricting systems that could finely tune local blood flow depending on neuronal activity changes at the cerebellar input stage.
- Subjects
GRANULE cells; METHYL aspartate receptors; NITRIC oxide; CEREBELLUM; CAPILLARIES
- Publication
Journal of Neuroscience, 2017, Vol 37, Issue 5, p1340
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.2025-16.2016