We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
NUP210 and MicroRNA-22 Modulate Fas to Elicit HeLa Cell Cycle Arrest.
- Authors
Qiao Gu; Wenjie Hou; Huan Liu; Lijuan Shi; Zonghao Zhu; Wenfeng Ye; Xiaoyuan Ni
- Abstract
Purpose: Cervical cancer is one of the most fatal diseases among women in under-developed countries. To improve cervical cancer treatment, discovery of new targets is needed. In this study, we investigated the expression of NUP210, miR-22, and Fas in cervical cancer tissues and their functions in cell cycle regulation. Materials and Methods: We detected and compared the expression levels of NUP210, miR-22, and Fas in cervical cancer tissues with paired normal tissues using immunohistochemistry, Western blot, and real-time quantitative polymerase chain reaction. NUP210 was knocked down in HeLa cells via lentivirus, followed by cell cycle and proliferation analysis. Using a luciferase reporter assay, we explored the link between miR-22 and NUP210. We overexpressed miR-22 in HeLa cells and analyzed cell cycle and proliferation function. We then overexpressed miR-22 in NUP210 knockdown cells to explore the connection between Fas and miR- 22-NUP210 signaling. Results: We found that NUP210 was overexpressed in cervical cancer patients. Knocking down NUP210 restored cell apoptosis and proliferation. We confirmed miR-22 as a regulator of NUP210 and verified that miR-22 was inhibited in cervical cancer development. We also found that restoring miR-22 expression could induce cell apoptosis. Finally, we found that miR-22-regulated expression of NUP210 could alter Fas expression and, in turn, elicit cell cycle arrest and proliferation. Conclusion: miR-22 in cervical cancer is downregulated, resulting in NUP210 overexpression and inhibition of Fas-induced cell apoptosis.
- Subjects
CELL cycle; HELA cells; CELL cycle regulation; CERVICAL cancer; CELL physiology
- Publication
Yonsei Medical Journal, 2020, Vol 61, Issue 5, p371
- ISSN
0513-5796
- Publication type
Article
- DOI
10.3349/ymj.2020.61.5.371