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- Title
Ananas comosus L. Leaf Phenols and p-Coumaric Acid Regulate Liver Fat Metabolism by Upregulating CPT-1 Expression.
- Authors
Weidong Xie; Shaobo Zhang; Fan Lei; Xiaoxi Ouyang; Lijun Du
- Abstract
In this study, we aimed to investigate the effect and action mechanisms of pineapple leaf phenols (PLPs) on liver fat metabolism in high-fat diet-fed mice. Results show that PLP significantly reduced abdominal fat and liver lipid accumulation in high-fat diet fed mice. The effects of PLP were comparable with those of FB. Furthermore, at the protein level, PLP upregulated the expression of carnitine palmitoyltransferase 1 (CPT-1), whereas FB had no effects on CPT-1 compared with the HFD controls. Regarding mRNA expression, PLP mainly promoted the expression of CPT-1, PGC1a, UCP-1, and AMPK in the mitochondria, whereas FB mostly enhanced the expression of Ech1, Acox1, Acaa1, and Ehhadh in peroxisomes. PLP seemed to enhance fat metabolism in the mitochondria, whereas FB mainly exerted the effect in peroxisomes. In addition, p-coumaric acid (CA), one of the main components from PLP, significantly inhibited fat accumulation in oleic acid-induced HepG2 cells. CA also significantly upregulated CPT-1 mRNA and protein expressions in HepG2 cells. We, firstly, found that PLP enhanced liver fat metabolism by upregulating CPT-1 expression in the mitochondria and might be promising in treatment of fatty liver diseases as alternative natural products. CA may be one of the active components of PLP.
- Subjects
FATTY liver prevention; LIVER analysis; ANALYSIS of variance; ANIMAL experimentation; BIOCHEMISTRY; BODY weight; CARNITINE; FAT; FATTY liver; FAT content of food; LEAVES; LIVER; MICE; MITOCHONDRIA; PINEAPPLE; POLYMERASE chain reaction; POLYPHENOLS; RESEARCH funding; WESTERN immunoblotting; PHYTOCHEMICALS; REVERSE transcriptase polymerase chain reaction; FLUOROIMMUNOASSAY; ABDOMINAL adipose tissue; THERAPEUTICS
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2014, Vol 2014, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2014/903258