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- Title
Salidroside Improves Homocysteine-Induced Endothelial Dysfunction by Reducing Oxidative Stress.
- Authors
Sin Bond Leung; Huina Zhang; Chi Wai Lau; Yu Huang; Zhixiu Lin
- Abstract
Hyperhomocysteinemia is associated with an increased risk for cardiovascular diseases through increased oxidative stress. Salidroside is an active ingredient of the root of Rhodiola rosea with documented antioxidative, antihypoxia and neuroprotective properties. However, the vascular benefits of salidroside against endothelial dysfunction have yet to be explored. The present study, therefore, aimed to investigate the protective effect of salidroside on homocysteine-induced endothelial dysfunction. Functional studies on the rat aortas were performed to delineate the vascular effect of salidroside. DHE imaging was used to evaluate the reactive oxygen species (ROS) level in aortic wall and endothelial cells. Western blotting was performed to assess the protein expression associated with oxidative stress and nitric oxide (NO) bioavailability. Exposure to homocysteine attenuated endothelium-dependent relaxations in rat aortas while salidroside pretreatment rescued it. Salidroside inhibited homocystein-induced elevation in the NOX2 expression and ROS overproduction in both aortas and cultured endothelial cells and increased phosphorylation of eNOS which was diminished by homocysteine. The present study shows that salidroside is effective in preserving the NO bioavailability and thus protects against homocysteine-induced impairment of endothelium-dependent relaxations, largely through inhibiting the NOX2 expression and ROS production. Our results indicate a therapeutic potential of salidroside in the management of oxidative-stress-associated cardiovascular dysfunction.
- Subjects
THERAPEUTIC use of plant extracts; ANALYSIS of variance; ANIMAL experimentation; AORTA; CARDIOVASCULAR diseases risk factors; CHEMILUMINESCENCE assay; ENDOTHELIUM; GENE expression; CHINESE medicine; MOUNTAIN sickness; RATS; RESEARCH funding; SODIUM nitroferricyanide; STATISTICS; T-test (Statistics); WESTERN immunoblotting; DATA analysis; OXIDATIVE stress; DATA analysis software; HYPERHOMOCYSTEINEMIA; DISEASE complications
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2013, Vol 2013, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2013/679635