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- Title
LQB-118, an orally active pterocarpanquinone, induces selective oxidative stress and apoptosis in Leishmania amazonensis.
- Authors
Ribeiro, Grazielle Alves; Cunha-Júnior, Edézio Ferreira; Pinheiro, Roberta Olmo; da-Silva, Silvia Amaral Gonçalves; Canto-Cavalheiro, Marilene Marcuzzo; da Silva, Alcides José Monteiro; Costa, Paulo Roberto R.; Netto, Chaquip Daher; Melo, Rossana C. N.; Almeida-Amaral, Elmo Eduardo; Torres-Santos, Eduardo Caio
- Abstract
Objectives The pterocarpanquinone LQB-118, previously demonstrated to be effective in vivo via oral delivery, was investigated for its mechanism in selective parasite killing. Methods Oxidative stress in Leishmania amazonensis was analysed by evaluating reactive oxygen species (ROS) production (2′,7′-dichlorodihydrofluorescein diacetate) and the loss of mitochondrial membrane potential (ΔΨm) using rhodamine, JC-1 and MitoCapture. Ultrastructural analysis was performed using transmission electron microscopy (TEM). DNA fragmentation was evaluated using terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL). Results Treatment with LQB-118 induced ROS production in the promastigotes of L. amazonensis in a concentration-dependent manner for the first 4 h and was sustained for 24 h. TEM analysis revealed several alterations typical of apoptosis. Promastigotes presented a reduction of ΔΨm after 24 h of incubation with 2.5 μM (18.7%), 5 μM (63.7%) or 10 μM (70.7%) LQB-118. A sub-G0/G1 cell cycle phenotype was observed in 21%–83% of the promastigotes incubated with 1.25–10 μM LQB-118. Concentration-dependent DNA fragmentation was observed in promastigotes treated with 2.5–10 μM LQB-118, and selective DNA fragmentation was observed in intracellular amastigotes after 72 h with 2.5 μM treatment. Conclusions Our results suggest that LQB-118 selectively induces ROS-triggered and mitochondria-dependent apoptosis in this parasite.
- Subjects
OXIDATIVE stress; DRUG delivery systems; LEISHMANIA; DIACETATES; PROMASTIGOTE; TRANSMISSION electron microscopy
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2013, Vol 68, Issue 4, p789
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dks498