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- Title
单细胞转录组测序技术发展及其在甘薯中的应用.
- Authors
赵 楠; 梁雪莲; 谢淑燕; 张新新; 马婷婷; 邹宏达; 陈景益; 房伯平; 黄立飞
- Abstract
Single-cell RNA-seq(scRNA-seq) technology is new to study overall gene expression at the single cell level by high-throughput sequencing of mRNA. This paper summarized the development and innovation of single cell transcriptome sequencing technology. The steps of single cell capture or sorting, reverse transcription/PCR amplification, database construction and sequencing data basic analysis of scRNA- seq technology were introduced in detaiband its application in the study of scRNA-seq technology in plant single cell map, abiotic stress response mechanism, cell differentiation and development, and transcription factor regulatory network were introduced. The application potential of single cell map, differentiation, stress resistance and gene in sweet potato was also analyzed. Finally, the application of this technology was prospected.
- Subjects
NUCLEOTIDE sequencing; GENE expression; CELL differentiation; ABIOTIC stress; DATABASES; SWEET potatoes
- Publication
Journal of Northwest A & F University - Natural Science Edition, 2023, Vol 51, Issue 3, p39
- ISSN
1671-9387
- Publication type
Article
- DOI
10.13207/j.cnki.jnwafu.2023.03.005