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- Title
Investigation of IFIT3 and KCNS3 Gene Expression Patterns in the Peripheral Blood of Cryptogenic Epilepsy Patients.
- Authors
ÖZCAN, Gülsima; KORKMAZ, Nur Damla; SÜSGÜN, Seda; YÜCESAN, Emrah; USLU, Ferda
- Abstract
Introduction: Epilepsy is a neurological disease, characterized by recurrent seizures. Cryptogenic epilepsies are defined as epilepsies with a lack of previous signs of brain damage and of obvious etiology. The absence of obvious causative pathology creates challenges in the clinical management of the disease. Gene expression studies aid in better clinical management, in terms of providing a better sight into etiology, or mechanisms leading to the disease. In this study, we studied the expression levels of IFIT3 and KCNS3 genes in blood samples to enlighten the molecular etiology of patients with cryptogenic epilepsy patients. Method: Our study includes cryptogenic epilepsy patients admissioning at Bezmialem Vakıf University (n=20) and healthy controls (n=20). The participants were all over the age of 18. Females and males were equally distributed in both groups. The peripheral blood samples were collected into EDTA tubes, and total RNAs were isolated immediately. Complementary DNAs (cDNAs) were synthesized from RNA samples within the approved range of purity. ACTB was designated as the housekeeping gene. Primers were designed for IFIT3, KCNS3, and ACTB. qRT-PCR was performed on cDNA samples of both patients and healthy controls. Results: Biostatistical analysis was conducted with Student t-test, using the delta delta Ct approach on Cycle of threshold (Ct) data. The expression levels of KCNS3 were higher in the patient group (p<0.05). IFIT3 levels didnot have a statistically significant difference between the two groups. Conclusion: Patient samples showed a higher expression of KCNS3, a potassium channel-related gene. Non-etheless, the IFIT3 gene, a gene functioning in immunity, didnot show any significant difference. Our findings suggest that a channelopathy is more likely to underly the disease, and that KCNS3 might have a role in the pathogenesis.
- Subjects
PEOPLE with epilepsy; GENE expression; EPILEPSY; COMPLEMENTARY DNA; NEUROLOGICAL disorders; BRAIN damage
- Publication
Bezmialem Science, 2023, Vol 11, pS5
- ISSN
2148-2373
- Publication type
Abstract