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- Title
Determination of Phytohormones in Plant Extracts Using In-matrix Ethyl Chloroformate Derivatization and DLLME—GC—MS.
- Authors
Shusheng Luo; Li Lin; Xiaowei Wang; Shichun Zou; Tiangang Luan
- Abstract
A fast and simple sample preparation method using in-matrix derivatization and dispersive liquid-liquid microextraction (DLLME) for the simultaneous determination of 11 phytohormones in plants by gas chromatography-mass spectrometry (GC-MS) was developed. In this derivatization-extraction procedure, phytohormones in aqueous samples were derivatized with ethyl chloroformate (ECF) and extracted by DLLME simultaneously using ethanol-pyridine (4:1, v:v), both as derivatization catalyst and DLLME dispersant. This proposed rapid and convenient method was also successfully applied for analysing the phytohormones in rice seed callus and cucumber fuits, indicating other wide applications in other plant tissues.
- Subjects
PLANT hormones; PLANT extracts; ETHYL chloride; DERIVATIZATION; GAS chromatography/Mass spectrometry (GC-MS); PLANT cells &; tissues
- Publication
LC-GC Europe, 2013, Vol 26, Issue 6, p310
- ISSN
1471-6577
- Publication type
Article