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- Title
The expression of murine cutaneous late phase reaction requires both IgE antibodies and CD4 T cells.
- Authors
Sawada, K.; Nagai, H.; Basaki, Y.; Yamaya, H.; Ikizawa, K.; Watanabe, M.; Kojima, M.; Matsuura, N.; Kiniwa, M.
- Abstract
Background Exposure of atopic patients to a specific allergen evokes an immediate response which is followed, in many cases. by a late phase reaction (LPR) some hours later. Here we have examined tile immunological mechanisms required for the expression of cutaneous LPR in mice. Methods BALB/c mice were immunized by i.p. injection of ovalbumin (OVA) and alum act ively or by i.v. injection of anti-OVA IgE monoclonal antibody (mAb) passively. After challenge by intradermal injection of OVA into ears, the changes in ear thickness, the number of eosinophils, and the levels of IL-4 and IFN-y protein a the site of antigen challenge were examined. Results Actively immunized mice developed a biphasic response at the site of OVA injection, while mice passively immunized with IgE anti-OVA mAb displayed a strong early response but no LPR. Cell transfer experiments using BALB/c nu/nu mice revealed that both OVA-specific IgE mAb and OVA-primed CD4 T cells were required to evoke LPR. Moreover, LPR was associated with increased levels of IL-4 production concomitant with reduced IFN-γ production and was abolished by pretreatment with anti-IL-4 neutralizing mAb. Conclusion It is suggested that murine cutaneous LPR against OVA is a type 2 inflammatory response in which both IgE antibodies and CD4 T cells play an obligatory role.
- Subjects
IMMUNOGLOBULIN E; LABORATORY mice; ALLERGENS; IMMUNOGLOBULINS; IMMUNIZATION; ANTIGENS
- Publication
Clinical & Experimental Allergy, 1997, Vol 27, Issue 2, p225
- ISSN
0954-7894
- Publication type
Article
- DOI
10.1111/j.1365-2222.1997.tb00697.x