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- Title
Variables pre-analíticas que afectan la cuantificación sérica de Proteína-C-Reactiva e IL-6 en primigestantes preeclámpticas y controles sanos.
- Authors
Castellano Castellanos, Yuly Andrea; Moreno Castañeda, Nathalia Fernanda; Villamizar Caballero, Harwing Yoneider; Gamboa Blanco, Liseth Marcela; Sus Carrizosa, Sara Elizabeth; Mahecha, Elizabeth Guio
- Abstract
Background: Preeclampsia is associated with an increase in the inflammatory response; two molecules associated with this process are the CRP and IL-6. In the detection of serum levels of these molecules, there are preanalytical factors that can modify its concentration. This study seeks to establish whether there is variability in levels of IL-6 and CRP according to the times of separation and storage temperature on samples of preeclamptic primíparas and healthy controls. Methods: Peripheral blood was drawn using two tubes (A and B) of 7 ml without anticoagulant. Tube A was cooled to 4 °C for 7 days, at the end was centrifuged and stored at -70 °C. Tube B was centrifuged immediately and fractionated, the first fraction (Bl) was stored at -70 ° C (reference tube), while others were refrigerated at 4 ° C and frozen at -70 ° C after 15 days, one, two and three months. Finally, we quantified the serum levels of IL-6 and CRP. Results: 75 samples were quantified for CRP and 58 for IL-6. Differences in CRP concentrations found given by the delay of centrifugation after 7 days of storage at 4 °C. With regarding to IL 6, there were no significant differences. Conclusion: CRP and IL-6, may vary by exposure to preanalytical variables. In this study corroborate that serum CRP levels are significantly altered if the sample is centrifuged without be chilled; for the other variables analyzed showed no change in the quantification.
- Subjects
C-reactive protein; BLOOD serum analysis; INTERLEUKIN-6; PREECLAMPSIA; TEMPERATURE effect; BLOOD testing
- Publication
Revista Salud UIS, 2012, Vol 44, Issue 3, p25
- ISSN
0121-0807
- Publication type
Article