We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Rapid electrochemical detection of coronavirus SARS-CoV-2.
- Authors
Chaibun, Thanyarat; Puenpa, Jiratchaya; Ngamdee, Tatchanun; Boonapatcharoen, Nimaradee; Athamanolap, Pornpat; O'Mullane, Anthony Peter; Vongpunsawad, Sompong; Poovorawan, Yong; Lee, Su Yin; Lertanantawong, Benchaporn
- Abstract
Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/μL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19. Currently the most common method of COVID-19 diagnosis is by qRT-PCR which is slow and requires expensive instrumentation. Here the authors report an electrochemical biosensor based on isothermal rolling circle amplification for rapid detection of SARS-CoV-2 in clinical samples.
- Subjects
AMPLIFICATION reactions; COVID-19; SARS-CoV-2; COVID-19 testing; COMMUNICABLE diseases; CLASSICAL swine fever
- Publication
Nature Communications, 2021, Vol 12, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-021-21121-7