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- Title
Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohorts.
- Authors
Cardozo, Karina Helena Morais; Lebkuchen, Adriana; Okai, Guilherme Gonçalves; Schuch, Rodrigo Andrade; Viana, Luciana Godoy; Olive, Aline Nogueira; Lazari, Carolina dos Santos; Fraga, Ana Maria; Granato, Celso Francisco Hernandes; Pintão, Maria Carolina Tostes; Carvalho, Valdemir Melechco
- Abstract
The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is pressing public health systems around the world, and large population testing is a key step to control this pandemic disease. Here, we develop a high-throughput targeted proteomics assay to detect SARS-CoV-2 nucleoprotein peptides directly from nasopharyngeal and oropharyngeal swabs. A modified magnetic particle-based proteomics approach implemented on a robotic liquid handler enables fully automated preparation of 96 samples within 4 hours. A TFC-MS system allows multiplexed analysis of 4 samples within 10 min, enabling the processing of more than 500 samples per day. We validate this method qualitatively (Tier 3) and quantitatively (Tier 1) using 985 specimens previously analyzed by real-time RT-PCR, and detect up to 84% of the positive cases with up to 97% specificity. The presented strategy has high sample stability and should be considered as an option for SARS-CoV-2 testing in large populations. Large population testing is a key step to controlling the COVID-19 pandemic. Here, the authors develop a targeted mass spectrometry system for peptide-based SARS-CoV-2 detection, allowing analysis of over 500 swab samples per day and enabling virus detection even after prolonged sample storage at room temperature.
- Subjects
SARS-CoV-2; COVID-19 pandemic; COVID-19; MAGNETIC particles
- Publication
Nature Communications, 2020, Vol 11, Issue 1, pN.PAG
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-020-19925-0