We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Requirement of Cellular Protein CCT7 for the Replication of Fowl Adenovirus Serotype 4 (FAdV-4) in Leghorn Male Hepatocellular Cells Via Interaction with the Viral Hexon Protein.
- Authors
Gao, Junfeng; Zhao, Mingliang; Duan, Xueyan; Wang, Yongqiang; Cao, Hong; Li, Xiaoqi; Zheng, Shijun J.
- Abstract
Fowl adenovirus serotype 4 (FAdV-4) causes hepatitis-hydropericardium syndrome (HHS), leading to severe economic losses in the poultry industry. Although the pathogenesis of FAdV-4 infection has caused much attention, the underlying molecular mechanisms remain poorly understood. Here, we identified chaperonin containing TCP-1 subunit eta (CCT7) as an interacting partner of the FAdV-4 capsid protein hexon. We found that ectopic expression of CCT7 in leghorn male hepatocellular (LMH) cells enhanced hexon expression in pRK5-flag-hexon transfected cells. On the contrary, knockdown of cellular CCT7 by RNAi markedly reduced hexon expression in FAdV-4-infected cells and suppressed viral replication. These data suggest that CCT7 is required for FAdV-4 replication and may serve as a potential target for controlling FAdV-4 infection.
- Subjects
ADENOVIRUSES; CANCER cells; HEPATITIS; MOLECULAR chaperones; LIVER cancer
- Publication
Viruses (1999-4915), 2019, Vol 11, Issue 2, p107
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v11020107