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- Title
Serodiagnosis of invasive amebiasis using a recombinant Entamoeba histolytica protein.
- Authors
Stanley, S L Jr; Jackson, T F; Reed, S L; Calderon, J; Kunz-Jenkins, C; Gathiram, V; Li, E
- Abstract
One hundred eight serum samples from 106 patients were examined by Western blot analysis for the presence of antibodies to a recombinant fusion protein containing the sequence of the newly described serine-rich Entamoeba histolytica protein (SREHP). Among patients with invasive amebiasis from Durban, Republic of South Africa; San Diego, Calif; Mexico City, Mexico; and St Louis, Mo, 53 (82%) of 65 had antibodies to SREHP. In contrast, only one patient (2%) of 43 without acute invasive amebiasis had antibodies to SREHP. The predictive value of a positive test for anti-SREHP antibodies in the detection of acute invasive amebiasis was most marked when analyzed in the patients from Durban, where 11 (92%) of 12 patients who were seropositive for SREHP had acute invasive amebiasis vs 17 (65%) of 26 patients who had a positive serologic diagnosis as determined by agar gel diffusion. The use of a serologic test based on the recombinant SREHP fusion protein may be a useful adjunct to the diagnosis of acute invasive amebiasis in endemic regions.
- Subjects
AMEBIASIS; AMEBIC liver abscess; ANIMAL experimentation; COMPARATIVE studies; RESEARCH methodology; MEDICAL cooperation; PROTEINS; PROTOZOA; RECOMBINANT proteins; RESEARCH; SERODIAGNOSIS; EVALUATION research; DIAGNOSIS
- Publication
JAMA: Journal of the American Medical Association, 1991, Vol 266, Issue 14, p1984
- ISSN
0098-7484
- Publication type
journal article
- DOI
10.1001/jama.266.14.1984