We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A Small RNA Encoded in the Rv2660c Locus of <i>Mycobacterium tuberculosis</i> Is Induced during Starvation and Infection.
- Authors
Houghton, Joanna; Cortes, Teresa; Schubert, Olga; Rose, Graham; Rodgers, Angela; De Ste Croix, Megan; Aebersold, Rudolf; Young, Douglas B.; Arnvig, Kristine B.
- Abstract
Enhanced transcription of the Rv2660c locus in response to starvation of Mycobacterium tuberculosis H37Rv encouraged addition of the predicted Rv2660c protein to an improved vaccine formulation. Using strand-specific RNA sequencing, we show that the up-regulated transcript is in fact a small RNA encoded on the opposite strand to the annotated Rv2660c. The transcript originates within a prophage and is expressed only in strains that carry PhiRv2. The small RNA contains both host and phage sequences and provides a useful biomarker to monitor bacterial starvation during infection and/or non-replicating persistence. Using different approaches we do not find any evidence of Rv2660c at the level of mRNA or protein. Further efforts to understand the mechanism by which Rv2660c improves efficacy of the H56 vaccine are likely to provide insights into the pathology and immunology of tuberculosis.
- Subjects
NON-coding RNA; GENETIC transcription in bacteria; STARVATION; VACCINES; NUCLEOTIDE sequence; GENE expression; MYCOBACTERIUM tuberculosis; BACTERIA
- Publication
PLoS ONE, 2013, Vol 8, Issue 12, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0080047