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- Title
Three‐Dimensional MAX‐Ti<sub>3</sub>AlC<sub>2</sub> Nanomaterials for Dual‐Selective and Highly Efficient Enrichment of Phosphorylated and Glycosylated Peptides.
- Authors
Yang, Shi‐Shu; Wang, Chen; Jiang, Yu‐Fei; Zhang, Hua
- Abstract
Dual‐selective enrichment of phosphopeptides and glycopeptides of post‐translational modifications (PTMs) in the complex biological samples are challenging. In this work, considering the versatile properties including surface abundant metal sites and electrostatic attraction between Ti3C2‐layers and Al‐layers, layered ternary carbides Ti3AlC2 nanomaterials was successfully applied for the first time as an affinity adsorbent for the dual‐selective capture of phosphopeptides and glycopeptides. Especially, the Ti3AlC2 nanomaterials had an excellent detection sensitivity for phosphopeptides (1×10−11 M) and a good selectivity for glycopeptides with a low molar ratio of 1 : 500 of HRP (horseradish peroxidase) to BSA (bovine serum albumin). Furthermore, Ti3AlC2 nanomaterials was also applied for dual‐selective enrichment of phosphopeptides and glycopeptides from mouse brain neocortex lysate and human serum lysate respectively before mass spectrometry (MS) analysis, yielding twenty‐two unique phosphopeptides from thirteen phosphoproteins and fifty‐three unique glycopeptides from thirty‐seven glycoproteins, respectively. This work will open a new avenue and will greatly promote sample preparation for mass spectrometric analysis in phosphoproteomics and glycoproteomics research.
- Subjects
PEPTIDES; PHOSPHOPEPTIDES; PHOSPHOPROTEINS; NANOSTRUCTURED materials; GLYCOPROTEINS
- Publication
ChemPlusChem, 2023, Vol 88, Issue 1, p1
- ISSN
2192-6506
- Publication type
Article
- DOI
10.1002/cplu.202200375