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- Title
QUALITATIVE DETERMINATION OF ALLELOCHEMICALS IN Psidium guajava L. LEAVES FOR THEIR EFFECT AGAINST WHEAT AND CANARY GRASS.
- Authors
Yousaf, Muhammad; Zahoor, Ameer Fawad; Ali, Kulsoom Ghulam; Anjum, Anbreen; Shahzadi, Hina; Mubeen, Zunaira; Awais, Muhammad; Purveen, Shagufta
- Abstract
Excessive use of herbicides in fields threatens the environment by destroying flora and fauna. Allelopathic substances might be proved as a substitute of chemical herbicides to suppress the target weeds. In this study, the Psidium guajava L. leaves were extracted with 70% (v/v) methanol for the constitutional composition. The allelopathic effects were investigated against pre- and postgerminated wheat and canary grass seeds. Among the evaluated 16 allelochemicals, some eight components (Ellagic acid, 3,4-dihydroxybenzoic acid, Feruic acid, Galluic acid, Vivillic acid, 3-hydroxy-benzoic acid, Gallic acid and p-coumaric acid) were identified by comparing the chromatograms of the unknown with standard ones. The pre- and post-germinated bioassays were taken into consideration for wheat and canary grass separately. The results showed that over all 70% methanol extract of P. guajava L. leaves (2.5-10%) increased the shoot length and root linearly for both pre-germinated wheat bioassays (14.0 - 16.5cm and 12.9 - 15.0cm and post germinated wheat bioassays 13.85 - 16.25 cm and 11.15 - 14.50 cm, respectively) as compared with control ones. Similarly, allelochemicals of P. guajava L. suppressed both the pregerminated and post-germination bioassays of canary grass as compared with control ones (7.35 - 0.45cm and 6.50 - 2.75cm for shoot and 1.65 - 0.05cm and 2.15 - 0.75cm for root, respectively). These results suggest that P. guajava L. may contain growth inhibitory substances and may possess allelopathic potential. Therefore, leaves of P. guajava L. may be a possible candidate for the isolation and identification of allelopathic substances and for the development of natural herbicides for sustainable agricultural production.
- Subjects
HERBICIDES &; the environment; ALLELOPATHIC agents; WEED control; GUAVA; PHALARIS canariensis; ALLELOCHEMICALS; GALLIC acid
- Publication
Pakistan Journal of Weed Science Research, 2013, Vol 19, Issue 3, p239
- ISSN
1815-1094
- Publication type
Article