We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of acanthoic acid on TNF- alpha gene expression and haptoglobin synthesis.
- Authors
Kang, H-S.; Song, H. K.; Lee, J-J.; Pyun, K-H.; Choi, I.
- Abstract
TUMOUR necrosis factor- alpha (TNF- alpha ) is a major proinflammatory cytokine inducing the synthesis and release of many inflammatory mediators. It is involved in immune regulation, autoim mune diseases, and inflammation. Our previous study demonstrated that acanthoic acid, (-)-pimara-9(11), 15-dien19-oic acid, a pimaradiene diterpene isolated from Acanth op anax ko reanum , inhibited TNF- alpha production. To extend our understanding of inhibitory effects of acanthoic acid on TNF- alpha production, its effects on TNF- alpha gene expression was tested. Based on the results from RT-PCR and promoter analysis of TNF- alpha , it was found that acanthoic acid suppressed TNF- alpha gene expression. But the same concentration of acanthoic acid had no effect on IL-6 gene expression. Haptoglobin is an acute phase protein which is induced by TNF- alpha . When liver cells were treated with acanthoic acid, haptoglobin synthesis was blocked by acanthoic acid. These data confirmed that acanthoic acid inhibited gene expression and biological function of TNF- alpha .
- Subjects
TUMOR necrosis factors; GENE expression
- Publication
Mediators of Inflammation, 1998, Vol 7, Issue 4, p257
- ISSN
0962-9351
- Publication type
Article
- DOI
10.1080/09629359890938