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- Title
Critical re-appraisal of blood component quality after overnight hold of whole blood outside current room temperature limits.
- Authors
Bontekoe, I. J.; Meer, P. F.; Korte, D.
- Abstract
Background and Objectives According to European guidelines, the temperature of whole blood ( WB) has to be maintained at 20-24°C until processing within 24 h, but in blood bank practice, WB is frequently held at temperatures between 18-25°C. We aimed to assess the impact of these small temperature deviations on the quality of the blood components. Materials and Methods After rapid cooling, 7 WB units were held overnight at 18°C and 8 units at 25°C, reflecting worst case holding conditions, and separated into a red cell concentrate ( RCC), plasma and buffy coat ( BC). RCCs were filtered at test temperature and stored for 42 days at 2-6°C. BCs were processed to single- BC platelet concentrates ( sPC) and stored up to Day 8 at 20-24°C. Results After overnight hold at 18°C, 2,3- DPG in WB decreased by 34 ± 9%, while at 25°C the decrease was 82 ± 6%. Accordingly, the 2,3- DPG levels in the RCCs in the 25°C group were significantly lower than in the 18°C group (2·2 ± 1·4 vs. 10·4 ± 2·9 μmol/g Hb). RCCs and sPCs in the 25°C group showed higher initial lactate levels and lower pH compared to the 18°C group, but these differences levelled off at the end of storage. RCCs showed small differences in ATP levels and haemolysis. Plasma in both groups showed comparable Factor VIII:C levels. Conclusion The temperature of WB during overnight hold strongly affects initial 2,3- DPG levels of RCCs and supports the maintenance of temperature limits between 20 and 24°C. Other in vitro effects of the temperature deviations were small and of no practical relevance.
- Subjects
BLOOD platelets; ERYTHROCYTES; BLOOD products; BUFFY coat; TEMPERATURE measurements
- Publication
Vox Sanguinis, 2017, Vol 112, Issue 2, p105
- ISSN
0042-9007
- Publication type
Article
- DOI
10.1111/vox.12474