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- Title
Evaluation of a universal point-of-issue assay for bacterial detection in buffy coat platelet components.
- Authors
Ramirez‐Arcos, S.; Kou, Y.; Perkins, H.
- Abstract
Bacterial contamination of platelet concentrates poses a major post-transfusion infectious risk. This study was aimed at evaluating the efficacy of the BacTx ® assay (Immunetics Inc.) for bacterial detection in leucocyte-reduced buffy coat platelet pools and for its sensitivity in detecting clinical isolates, including bacteria that form surface-attached aggregates (biofilm positives). Platelet pools were inoculated at bacterial concentrations of 0·8-13 CFU/ml. The BacTx ® assay detected all species at concentrations ≥103 CFU/ml within 20-69 h of platelet incubation. Detection of slow-growing and biofilm-forming strains was delayed in comparison with the other strains. This assay could be used as a point-of-issue method to increase the safety of the platelet supply.
- Subjects
BUFFY coat; BLOOD platelets; BLOOD transfusion; LEUCOCYTES; BLOOD testing
- Publication
Vox Sanguinis, 2014, Vol 107, Issue 2, p192
- ISSN
0042-9007
- Publication type
Article
- DOI
10.1111/vox.12148