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- Title
Bacterial Lipopolysaccharides Bind Selectively to Lymphocytes from Lipopolysaccharide High--responder Mouse Strains.
- Authors
Nygren, H.; Dahlén, G.; Möller, G.
- Abstract
Three different concentrations of horseradish peroxidase-labelled lipopolysaccharide (LPS-HRP) were added in vitro to spleen cells from the LPS high-responder strain C3H/Tif and to cells from the low-responder strain C3H/HeJ. After being washed and fixed the cells were exposed to the substrate and prepared for electron microscopy. After addition of 7 and 0.7 μg/ml of labelled LPS only lymphocytes from the high-responder strain were labelled. About 5–10 % of the cells from C3H/Tif bound LPS, which is in accordance with the known frequency of B cells possessing the genetically determined LPS receptor. At the highest dose of labelled LPS (70 μg/ml) a large proportion of lymphocytes from the low-responder strain also bound LPS. Erythrocytes from both strains bound LPS at all concentrations. It is concluded that LPS-HRP allows the detection at the cellular level of LPS binding to the genetically controlled membrane receptor for LPS.
- Subjects
BACTERIAL toxins; LYMPHOCYTES; MICROBIAL polysaccharides; ENDOTOXINS; ELECTRON microscopy; CELLULAR immunity
- Publication
Scandinavian Journal of Immunology, 1979, Vol 10, Issue 6, p555
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1979.tb01390.x