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- Title
Lymphocyte Associated β<sub>2</sub>-Microglobulin Studied by Crossed Radioimmunoelectrophoresis.
- Authors
Plesner, T.
- Abstract
A sensitive radioimmunoelectrophoretic assay was employed in the study of β2-microglobulin (β2m corresponding to 3 x 106 lymphocytes. The intrisic (amphiphilic) membrane proteins were solubilized and examined in crossed immunoelectrophoresis against rabbit antisera in the presence of non-ionic detergent. The β2m associated precipitate was traced by post-electrophoretic incubation with 125I-labelled antibodies to β2m and autoradiography. A polyspecific rabbit antiserum to human lymphoid cells retained its capacity to precipitate lymphocyte β2m after absorption with isolated β2m (on an immunosorbent column), showing that lymphocyte β2m is complexed to other molecules. No free β2m was found on lymphocytes when examined against the absorbed anti-serum to human lymphoid cells in an intermediate gel and anti-β2m in a reference gel.
- Subjects
LYMPHOCYTES; IMMUNOELECTROPHORESIS; IMMUNODIAGNOSIS; MEMBRANE proteins; LYMPHOID tissue; IMMUNE serums
- Publication
Scandinavian Journal of Immunology, 1978, Vol 8, Issue 4, p363
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1978.tb00530.x