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- Title
In Situ Monitoring of Aptamer–Protein Binding on a ZnO Surface Using Spectroscopic Ellipsometry.
- Authors
Alshammari, Adeem; van Zalinge, Harm; Sandall, Ian
- Abstract
The dissolution of zinc oxide is investigated using spectroscopic ellipsometry to investigate its suitability as a platform for biosensing applications. The results indicate that once the ZnO surface has been functionalised, it is suitably protected, and no significant dissolving of the ZnO occurs. The binding kinetics of the SARS-CoV-2 spike protein on aptamer-functionalised zinc oxide surfaces are subsequently investigated. Values are extracted for the refractive index and associated optical constants for both the aptamer layer used and the protein itself. It is shown that upon an initial exposure to the protein, a rapid fluctuation in the surface density is observed. After around 20 min, this effect stabilises, and a fixed increase in the surface density is observed, which itself increases as the concentration of the protein is increased. This technique and setup are demonstrated to have a limit-of-detection down to 1 nanomole (nM) and display a linear response to concentrations up to 100 nM.
- Subjects
ELLIPSOMETRY; ZINC oxide; OPTICAL constants; REFRACTIVE index; ZINC proteins
- Publication
Sensors (14248220), 2023, Vol 23, Issue 14, p6353
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s23146353