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- Title
DNA methylome analysis using short bisulfite sequencing data.
- Authors
Krueger, Felix; Kreck, Benjamin; Franke, Andre; Andrews, Simon R
- Abstract
Bisulfite conversion of genomic DNA combined with next-generation sequencing (BS-seq) is widely used to measure the methylation state of a whole genome, the methylome, at single-base resolution. However, analysis of BS-seq data still poses a considerable challenge. Here we summarize the challenges of BS-seq mapping as they apply to both base and color-space data. We also explore the effect of sequencing errors and contaminants on inferred methylation levels and recommend the most appropriate way to analyze this type of data.
- Subjects
DNA analysis; METHYLATION; GENE mapping; GENOMIC imprinting; GENETIC genealogy
- Publication
Nature Methods, 2012, Vol 9, Issue 2, p145
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1828