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- Title
Single molecule–sensitive probes for imaging RNA in live cells.
- Authors
Santangelo, Philip J.; Lifland, Aaron W.; Curt, Paul; Sasaki, Yukio; Bassell, Gary J.; Lindquist, Michael E.; Crowe, James E.
- Abstract
To visualize native or non-engineered RNA in live cells with single-molecule sensitivity, we developed multiply labeled tetravalent RNA imaging probes (MTRIPs). When delivered with streptolysin O into living human epithelial cancer cells and primary chicken fibroblasts, MTRIPs allowed the accurate imaging of native mRNAs and a non-engineered viral RNA, of RNA co-localization with known RNA-binding proteins, and of RNA dynamics and interactions with stress granules.
- Subjects
RNA; NUCLEIC acids; CELLULAR pathology; CANCER cells; CONNECTIVE tissue cells; CARRIER proteins
- Publication
Nature Methods, 2009, Vol 6, Issue 5, p347
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1316