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- Title
Anti-Radical Activity and Total Polyphenol Content in Selected Pure and Commercial Honey.
- Authors
Rahmat, Asmah; Abu Bakar, Mohd Fadzelly
- Abstract
BACKGROUND Researchers have found that honey maybe a healthier alternative to corn syrup due to its higher level of antioxidants, compounds which are believed to fight cancer, heart disease and other diseases. MATERIAL AND METHODS Four different types of honey were selected for this study; 'madu lebah asli' (Pahang) which contained 100% of pure honey, 'madu lebah tualang' (N'apis) which also contained 100% of pure honey, 'cuka madu asli' (Biofeld) and 'madu abang power' (N'apis) which contained the mixture of honey and selected herbs such as 'tongkat ali', 'ubi jaga' and 'penawar pahit'. DPPH free radical scavenging assay and Folin-Ciocalteau method were applied for determination of anti-radical activitiy and total polyphenol content of the samples, respectively. The anti-radical activity and total polyphenol content were expressed as mean of triplicate values and anti-radical activity was compared to synthetic antioxidant, butylated hydroxyl toluene (BHT). One way ANOVA test (p<0.05) was used to determine the significant differences between samples. RESULTS The DPPH free radical scavenging activity of BHT at concentration of 200 mg/l was the highest (76.76%), followed by 'madu abang power' (N'apis) (55.17%), BHT at concentration of 50 mg/l (43.95%), 'madu lebah tualang' (N'apis) (33.39%), 'madu lebah asli' (Pahang) (30.89%) and 'cuka madu asli' (Biofeld) (29.79%). The same trend was also observed in the total polyphenol content. Among the honey samples tested, the total polyphenol content in 'madu abang power' (N'apis) was the highest, followed by 'madu lebah tualang' (N'apis) and 'madu lebah asli' (Pahang). Total polyphenol content was least in 'cuka madu asli' (Biofeld). The values were 1.46, 0.60, 0.33 and 0.13 mg GAE / 10 g, respectively. CONCLUSION The anti-radical activity and total polyphenol content were higher in the mixture of honey and selected herbs (madu abang power) when compared to 100% of pure honey (madu lebah tualang and madu lebah asli. Cuka madu asli has the lowest anti-radical activity and total polyphenol content. This may be due to vinegar formation.
- Subjects
HONEY; POLYPHENOLS; ANTIOXIDANTS; HERBS; VINEGAR
- Publication
Malaysian Journal of Medical Sciences, 2007, Vol 14, Issue 1, p121
- ISSN
1394-195X
- Publication type
Article