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- Title
Cleavable, Covalently Linked, Affinity Coupling Immune Magnetic Nanoparticles for Specifically Depleting T Cells.
- Authors
Huynh, Kien-Quang; Duong, Duong Thai-Song; Van Tran, Thuan; Nguyen, Bich-Tram Thi; Tran-Nguyen, Thao-Suong; Tran, Thuoc Linh; Phan, Thang Bach; Ta, Kieu-Hanh Thi; Tran-Van, Hieu
- Abstract
Hematopoietic stem cell (HSC) transplantation has become an accepted therapeutic modality for patients with hematopoietic disorders. However, transplant recipients are at high risk of a severe complication known as graft versus host disease (GvHD). To reduce the risk of GvHD, depletion of T cells in donor HSCs prior to transplantation is of vital importance. Magnetic-activated cell sorting (MACS) using immuno-magnetic nanoparticles to selectively deplete T cells presents a potential solution for HSC transplantation. In this study, we presented a complete protocol for the synthesis of immuno-magnetic nanoparticles and characterised their performance for the depletion of Jurkat T cells from cell culture and from a mixture of T cells and HSCs. First, the recombinant protein A/G, an Fc-specific antibody, and magnetic nanoparticles were covalently linked by amine groups on the surface of magnetic nanoparticles and the protein using 3-(2-pyridyldithio) propionic acid N-hydroxysuccinimide ester (SPDP). Then, the anti-Jurkat T antibodies were bound to protein A/G on magnetic nanoparticles via regio-oriented affinity binding. Approximately 85 μg of protein A/G and 25 μg of antibody were bound to 1 mg of magnetic beads. The immuno-magnetic nanoparticles were capable of depleting up to 73% of Jurkat T cells from culture medium and 72% of Jurkat T cells from the mixture. In addition, we showed that our depletion protocol was able to preserve the proliferation and differentiation characteristics of HSCs. In conclusion, our immuno-magnetic nanoparticles provide a potential solution for the depletion of T-cells prior to HSCs transplantation.
- Subjects
MAGNETIC nanoparticles; T cells; MAGNETIC coupling; GRAFT versus host disease; HEMATOPOIETIC stem cells
- Publication
Journal of Electronic Materials, 2020, Vol 49, Issue 11, p6510
- ISSN
0361-5235
- Publication type
Article
- DOI
10.1007/s11664-020-08415-0