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- Title
An In Vitro Model for the Study of the Macrophage Response Upon Trichophyton rubrum Challenge.
- Authors
Yoshikawa, Fabio; Ferreira, Lucas; Almeida, Fernando; Almeida, Sandro
- Abstract
Diversity in the macrophage models currently employed in immunology studies may lead to opposed results and interpretations. In this study, we aimed to analyze the suitability of J774 macrophage-like cells as a model for the interaction between the dermatophyte Trichophyton rubrum and macrophages. J774 cells were competent in fungal phagocytosis, but succumbed to hyphal growth. Nevertheless, they could also secrete IL-1β in response to the dermatophyte. On the opposite direction, inflammatory, thioglycollate-induced peritoneal macrophages did not succumb to fungal growth and showed no significant IL-1β production. The proteomic profiling of these cells uncovered vimentin and plastin-2 as proteins whose abundance was altered by the fungal interaction. Our study indicates that this cell line could be an interesting tool in the investigation of T. rubrum infection biology.
- Subjects
TRICHOPHYTON; MACROPHAGES; PHAGOCYTOSIS; INTERLEUKIN-1; FIMBRIN; VIMENTIN
- Publication
Mycopathologia, 2017, Vol 182, Issue 1/2, p241
- ISSN
0301-486X
- Publication type
Article
- DOI
10.1007/s11046-016-0077-5