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- Title
Marker-assisted breeding for TaALMT1, a major gene conferring aluminium tolerance to wheat.
- Authors
Soto-Cerda, B.; Inostroza-Blancheteau, C.; Mathías, M.; Peñaloza, E.; Zuñiga, J.; Muñoz, G.; Rengel, Z.; Salvo-Garrido, H.
- Abstract
Aluminium toxicity in acid soils is the main limitation to crop production worldwide. In wheat ( Triticum aestivum L.), the Al-activated malate transporter ( TaALMT1) gene located on chromosome 4DL is associated with malate efflux and Al-tolerance. To introgress Al-tolerance from the breeding line CAR3911 into the high yielding Al-sensitive cultivar Kumpa-INIA, phenotypic and molecular characterizations of gene/QTL underlying Al-tolerance in CAR3911 followed by marker-assisted backcrossing (MAS-BC) were undertaken. Al-tolerant backcross (BC) lines were selected using the functional marker ALMT1-4 designed immediately upstream of the TaALMT1 coding region. Foreground and background selections using ALMT1-4 and microsatellite markers were conducted. Linkage and sequence analyses suggest that the TaALMT1 gene could underly the Al-tolerance in CAR3911, possessing the same promoter type (V) as the Al-tolerant genotypes Carazinho and ET8. The MAS-BC strategy allowed the selection of Al-tolerant lines with the smallest introgressed region (6 cM) on 4D and the highest recurrent parent genome (RPG) (98 %) covering 2 194 cM of the wheat genome. The homozygous BC3F2 line named Kumpa-INIA- TaALMT1 expressed a 3-fold higher Al-tolerance than its isogenic line Kumpa-INIA at 40 μM Al in the hydroponic solution, and similarly to CAR3911 and Carazinho. The MAS-BC strategy was successful for the introgression of the TaALMT1 gene into Kumpa-INIA in only three BC generations, shortening the breeding cycle to 24 months, which promises to increase wheat production and a greater yield stability in the acid soils of Southern Chile.
- Subjects
CHILE; WHEAT genetics; EFFECT of aluminum on plants; GENETIC markers in plants; PLANT breeding; PLANT chromosomes; ACID soils
- Publication
Biologia Plantarum, 2015, Vol 59, Issue 1, p83
- ISSN
0006-3134
- Publication type
Article
- DOI
10.1007/s10535-014-0474-x