We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Application of fluorescence-based resistance gene analog analysis for genotyping plant genetic resources.
- Authors
,2Jianping Cheng; Jun Yan; Tamar Krugman; Tzion Fahima
- Abstract
Conventional approaches for detecting disease resistance gene analogs (RGAs) in plants are based on agarose gels or on polyacrylamide gel electrophoresis (PAGE) in combination with silver staining or radioactive labeling. A modified method for RGA analysis has been developed by using fluorescence-labeled primers for PCR amplifications. The amplified fragments are detected by denaturing PAGE using an automated laser fluorescence DNA sequencer and analyzed by fragment analysis software. This technique is not limited to specific plant species and is suitable for high-throughput genotyping plant genetic resources. We demonstrate here the efficiency of this method for comparison of RGA patterns in diverse plant species and for genotyping of natural populations of the wheat progenitor,Triticum dicoccoides.
- Subjects
DNA fingerprinting; NATURAL immunity; IDENTIFICATION; FORENSIC genetics techniques; IMMUNITY; BIOTECHNOLOGY
- Publication
Biotechnology Letters, 2005, Vol 27, Issue 2, p83
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-004-6933-2