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- Title
Fungal Diversity of Human Gut Microbiota Among Eutrophic, Overweight, and Obese Individuals Based on Aerobic Culture-Dependent Approach.
- Authors
Borges, Francis M.; de Paula, Thaís O.; Sarmiento, Marjorie R. A.; de Oliveira, Maycon G.; Pereira, Maria L. M.; Toledo, Isabela V.; Nascimento, Thiago C.; Ferreira-Machado, Alessandra B.; Silva, Vânia L.; Diniz, Cláudio G.
- Abstract
Fungi have a complex role in the intestinal tract, influencing health and disease, with dysbiosis contributing to obesity. Our objectives were to investigate fungal diversity in human gut microbiota among eutrophic, overweight, and obese. Epidemiological and nutritional information were collected from adult individuals, as well as stool samples processed for selective fungi isolation and identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (yeasts) or microculture (filamentous fungi). Further 18S rDNA sequencing was performed to confirm identification. The mean count of fungi was 241 CFU/g of feces. Differences in the population level of the filamentous fungi were observed within eutrophic and obese groups. Overall, 34 genera were identified. The predominant phylum was Ascomycota with 20 different genera, followed by Basidiomycota and Zygomycota. As for Ascomycota, the most prevalent species were <italic>Paecilomyces</italic> sp., <italic>Penicillium</italic> sp., <italic>Candida</italic> sp., <italic>Aspergillus</italic> sp., <italic>Fonsecaea</italic> sp., and <italic>Geotrichum</italic> sp. (76.39, 65.28, 59.72, 58.33, 12.50, and 9.72%, respectively). As for Basidiomycota, <italic>Trichosporon</italic> sp. and <italic>Rhodotorula</italic> sp. were the most prevalent (30.56 and 15.28%, respectively), and for Zygomycota, <italic>Rhizopus</italic> sp. and <italic>Mucor</italic> sp. were the most numerous (15.28 and 9.72%, respectively). As expected there is a mycobiota shift towards obesity, with slightly higher diversity associated to eutrophic individuals. This mycobiota shift seems also to be related to the nutritional behavior of the individuals, as observed that the macronutrients intake may be positively related to the different fungi occurrences. Other studies are needed to better understand relationships between mycobiota and obesity, which could be used in future obesity treatments.
- Subjects
HUMAN microbiota; OVERWEIGHT persons; GASTROINTESTINAL system; OBESITY; TIME-of-flight spectrometry
- Publication
Current Microbiology, 2018, Vol 75, Issue 6, p726
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-018-1438-8