We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Role of 14-3-3σ in resistance to cisplatin in non-small cell lung cancer cells.
- Authors
Cetintas, Vildan Bozok; Tetik, Aslı; Cok, Gursel; Kucukaslan, Ali Sahin; Kosova, Buket; Gunduz, Cumhur; Veral, Ali; Eroglu, Zuhal
- Abstract
The efficacies of chemotherapeutic agents are often limited by side effects and acquired drug resistance. We have investigated whether the differential expression pattern of 14-3-3σ affects cisplatin response in non-small cell lung cancer cell lines. Two pairs of parental/cisplatin resistant cell lines (A549/CRA549 and Calu1/CR-Calu1) and clinical lung cancer biopsy samples were analysed for 14-3-3σ expression. Cell viability was assessed by WST assay; and 14-3-3σ expression was suppressed by siRNA transfection. 14-3-3σ mRNA expression increased in CR-A549 and CR-Calu1 compared with their cisplatin-sensitive parental A549 and Calu1 cell lines. But when 14-3-3σ expression was suppressed, elevated cisplatin response was seen in A549 and CR-Calu1 cell lines. Increased 14-3-3σ expression might also account for reduced cisplatin response in vivo, since, 14-3-3σ expression in clinical biopsy samples obtained from lung cancer patients undergoing cisplatin-based chemotherapy significantly higher in the non-responder compared with the responder group. We therefore propose that increased 14-3-3σ expression is correlated with cisplatin response in non-small cell lung cancer cells; monitoring its expression might become useful in the future in predicting poor outcome to cisplatin treatment and/or the verification of acquired cisplatin resistance in lung cancer patients.
- Subjects
DRUG resistance in cancer cells; CISPLATIN; LUNG cancer treatment; CANCER cells; CANCER chemotherapy; GENE expression
- Publication
Cell Biology International, 2013, Vol 37, Issue 1, p78
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1002/cbin.10006