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- Title
Src homology 2 domain of overexpressed Lyn kinase is responsible for the acceleration of granulocyte colony-stimulating factor-induced neutrophilic nuclear lobulation
- Authors
Oka, Naomi; Suzuki, Akiko; Omura, Tomomi; Sakai, Hiroshi; Murakami, Hiroshi
- Abstract
Abstract: We have previously shown that the overexpression of a Src family kinase, Lyn, and its kinase-negative form, LynKN, in a granulocyte progenitor cell line, GM-I62M, accelerates neutrophilic nuclear lobulation when the cells are cultured in the presence of granulocyte colony-stimulating factor. In this study, we investigated the role of the Src homology 2 (SH2) and SH3 domains of Lyn in the accelerated induction of nuclear lobulation. In contrast to wild-type Lyn, the overexpression of its SH2 domain mutant did not induce the accelerated nuclear morphological changes, but the overexpressed SH3 domain mutant had the same effects as wild-type Lyn. Therefore, the SH2 domain of Lyn is responsible for the accelerated induction of neutrophilic nuclear lobulation upon G-CSF stimulation.
- Subjects
GRANULOCYTES; CELL culture; NEUTROPHILS; PHAGOCYTES
- Publication
Cell Biology International, 2006, Vol 30, Issue 6, p525
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1016/j.cellbi.2006.02.009