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- Title
FK506 ENHANCED OSTEOBLASTIC DIFFERENTIATION IN MESENCHYMAL CELLS
- Authors
Tang, Lin; Ebara, Sohei; Kawasaki, Satoshi; Wakabayashi, Shinji; Nikaido, Toshio; Takaoka, Kunio
- Abstract
Bone morphogenetic protein (BMP) is a bone-derived growth factor capable of promoting the differentiation of mesenchymal cells into osteogenic lineage pathways. Recently, immunosuppressants were reported to cause a moderate increase in osteoblastic differentiation in a rat osteoblast-like osteosarcoma cell line. If immunosuppressants can induce osteoblastic differentiation, it will be useful for bone tissue transplantation. We assessed the effect of immunosuppressants with or without BMP-4 on inducing osteoblastic differentiation in osteoblast-like and other mesenchymal cells. FK506, an immunosuppressant often used clinically, induced a dose- and time-dependent increase in alkaline phosphatase (ALP) activity, one of the markers of osteoblast differentiation, in cells derived from mesenchyma. In the presence of BMP-4, ALP activity, mRNA levels of ALP and osteocalcin increased. FK506 was found to not only stimulate osteoblastic differentiation, but also to enhance BMP-4 induced osteoblastic differentiation. These results suggest that FK506 promotes differentiation of osteoblastic cells.
- Subjects
BONE morphogenetic proteins; TRANSFORMING growth factors-beta; RAPAMYCIN
- Publication
Cell Biology International, 2002, Vol 26, Issue 1, p75
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1006/cbir.2001.0812