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- Title
Serum miR-23 and miR-150 Profiles as Biomarkers for Predicting Recurrence following Surgical Intervention in Colorectal Cancer Patients.
- Authors
Mahmoudivar, Saeid; Zarredar, Habib; Asadi, Milad; Zafari, Venus; Hashemzadeh, Shahriyar; Farzaneh, Rojin; Kermani, Touraj Asvadi
- Abstract
Background: MicroRNAs (miRNAs) play pivotal roles in post-transcriptional regulation of gene expression and have emerged as crucial regulators in cancer development, progression, and metastasis. This study aimed to assess the expression profiles of miR-23, miR-223, miR-1246, and miR-150 in serum samples obtained from colorectal cancer (CRC) patients before and three months after surgery, in comparison to a healthy control group, to explore their biomarker potential. Methods: A total of 50 blood samples were collected from patients with CRC (pre- and post-surgery), along with 50 samples from healthy controls. The relative expression levels of miR-23, miR-223, miR-1246, and miR-150 in the serum were quantified using quantitative real-time PCR. Results: Our findings revealed upregulated expression levels of miR-23, miR-1246, and miR-223, while miR-150 exhibited significant downregulation in the serum of CRC subjects compared to healthy controls. Receiver operating characteristic (ROC) analysis indicated that miR-23 and miR-150 could distinguish CRC cases from controls with relatively high accuracy. Moreover, three months postsurgery, miR-23, miR-1246, and miR-223 serum levels were downregulated, and miR-150 was significantly upregulated. However, no significant correlations were observed between serum levels of the studied genes and the clinical features of our patients. Conclusions: The serum levels of miR-23 and miR-150 hold promise as potential biomarkers for the diagnosis and prognosis of CRC.
- Subjects
GENETIC regulation; RECEIVER operating characteristic curves; COLORECTAL cancer; CARCINOGENESIS; BIOMARKERS
- Publication
Reports of Biochemistry & Molecular Biology, 2024, Vol 12, Issue 4, p540
- ISSN
2322-3480
- Publication type
Article
- DOI
10.61186/rbmb.12.4.540