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- Title
Construction of HGF-Displaying Yeast by Cell Surface Engineering.
- Authors
Shibasaki, Seiji; Nakatani, Yuki; Taketani, Kazuaki; Karasaki, Miki; Matsui, Kiyoshi; Ueda, Mitsuyoshi; Iwasaki, Tsuyoshi
- Abstract
Hepatocyte growth factor (HGF) has been investigated as a regulator for immune reactions caused by transplantation and autoimmune diseases and other biological functions. Previous studies demonstrated that cDNA-encoding HGF administration could inhibit acute graft-versus-host disease (GVHD) after treatment via hematopoietic stem cell transplantation. This study aimed to show the preparation of HGF protein on yeast cell surfaces to develop a tool for the oral administration of HGF to a GVHD mouse model. In this study, full-length HGF and the heavy chain of HGF were genetically fused with α-agglutinin and were successfully displayed on the yeast cell surface. This study suggested that yeast cell surface display engineering could provide a novel administration route for HGF.
- Subjects
CELL membranes; HEMATOPOIETIC stem cell transplantation; HEPATOCYTE growth factor; YEAST; GRAFT versus host disease
- Publication
Microorganisms, 2022, Vol 10, Issue 7, pN.PAG
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms10071373