We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The Dis1/Stu2/XMAP215 Family Gene Fg Stu2 Is Involved in Vegetative Growth, Morphology, Sexual and Asexual Reproduction, Pathogenicity and DON Production of Fusarium graminearum.
- Authors
Zhu, Yuanye; Zhang, Yuanshuai; Liu, Na; Ren, Weichao; Hou, Yiping; Duan, Yabing; Song, Xiushi; Zhou, Mingguo
- Abstract
The conserved Dis1/Stu2/XMAP215 microtubule association proteins (MAPs) family plays an important role in microtubule dynamics, nucleation, and kinetochore-microtubule attachments. However, function of Dis1/Stu2/XMAP215 homolog in plant pathogenic fungi has not been determined. Here, we identified and investigated the Dis1/Stu2/XMAP215 homolog (FGSG_10528) in Fusarium graminearum (Fg Stu2p). Co-localization experiment and co-immunoprecipitation (Co-IP) assay demonstrated that Fg Stu2p is a microtubule associated protein. Besides, Fg Stu2 could also interact with Fg γ-tubulin and presumed Fg Ndc80, which suggested that the Fg Stu2 gene might associate with microtubule nucleation and kinetochore-microtubule attachments like Dis1/Stu2/XMAP215 homologs in other species. Moreover, the Fg Stu2 promoter replacement mutants (Fg Stu2-Si mutants) produced twisted hyphae and decreased growth rate. Microscope examination further showed that the microtubule polymerization was reduced in Fg Stu2-Si mutants, which could account for the aberrant morphology. Although the microtubule polymerization was affected in Fg Stu2-Si mutants, the Fg Stu2-Si mutants didn't show highly increased sensitivity to anti-microtubule fungicide carbendazim (methyl benzimidazol-2-ylcarbamate [MBC]). In addition, the Fg Stu2-Si mutants exhibited curved conidia, decreased number of conidial production, blocked ability of perithecia production, decreased pathogenicity and deoxynivalenol (DON) production. Taken together, these results indicate that the Fg Stu2 gene plays a crucial role in vegetative growth, morphology, sexual reproduction, asexual reproduction, virulence and deoxynivalenol (DON) production of F. graminearum , which brings new insights into the functions of Dis1/Stu2/XMAP215 homolog in plant pathogenic fungi.
- Subjects
ASEXUAL reproduction; MICROTUBULES; GENE families; FUSARIUM; MORPHOLOGY; TUBULINS
- Publication
Frontiers in Microbiology, 2020, Vol 11, pN.PAG
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2020.545015