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- Title
Noncarrier naked antigen-specific DNA vaccine generates potent antigen-specific immunologic responses and antitumor effects.
- Authors
Chen, C.-A.; Chang, M.-C.; Sun, W.-Z.; Chen, Y.-L.; Chiang, Y.-C.; Hsieh, C.-Y.; Chen, S. M.; Hsiao, P.-N.; Cheng, W.-F.
- Abstract
Genetic immunization strategies have largely focused on the use of plasmid DNA with a gene gun. However, there remains a clear need to further improve the efficiency, safety, and cost of potential DNA vaccines. The gold particle-coated DNA format delivered through a gene gun is expensive, time and process consuming, and raises aseptic safety concerns. This study aims to determine whether a low-pressured gene gun can deliver noncarrier naked DNA vaccine without any particle coating, and generate similarly strong antigen-specific immunologic responses and potent antitumor effects compared with gold particle-coated DNA vaccine. Our results show that mice vaccinated with noncarrier naked chimeric CRT/E7 DNA lead to dramatic increases in the numbers of E7-specific CD8+ T-cell precursors and markedly raised titers of E7-specific antibodies. Furthermore, noncarrier naked CRT/E7 DNA vaccine generated potent antitumor effects against subcutaneous E7-expressing tumors and pre-established E7-expressing metastatic pulmonary tumors. In addition, mice immunized with noncarrier naked CRT/E7 DNA vaccine had significantly less burning effects on the skin compared with those vaccinated with gold particle-coated CRT/E7 DNA vaccine. We conclude that noncarrier naked CRT/E7 DNA vaccine delivered with a low-pressured gene gun can generate similarly potent immunologic responses and effective antitumor effects has fewer side effects, and is more convenient than conventional gold particle-coated DNA vaccine.Gene Therapy (2009) 16, 776–787; doi:10.1038/gt.2009.31; published online 9 April 2009
- Subjects
DNA; GENES; DNA vaccines; TUMORS; IMMUNOGLOBULINS
- Publication
Gene Therapy, 2009, Vol 16, Issue 6, p776
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/gt.2009.31