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- Title
Systemic co-administration of depsipeptide selectively targets transfection enhancement to specific tissues and cell types.
- Authors
Liu, Y.; Liggitt, D.; Fong, S.; Debs, R. J.
- Abstract
Depsipeptide, a histone deacetylase (HDAC) inhibitor, kills tumor cells much more effectively than normal cells, and can produce significant antitumor activity in human cancer patients. Depsipeptide also increases the expression of lipoplex-delivered genes in cultured tumor cells, as well as following direct intra-tumoral injection. We now show that co-intravenous (i.v.) injection of depsipeptide with polyethylenimine (PEI):DNA complexes significantly increases the expression of PEI-delivered genes in normal, as well as in tumor-bearing mice. At the tissue level, depsipeptide-mediated enhancement of gene expression was selectively targeted to the lung, liver and spleen. At the cellular level, depsipeptide significantly increased the expression of the i.v., PEI co-delivered wild-type human p53 gene in metastatic breast cancer cells, but not in adjacent normal cells. Thus, the ability of depsipeptide to enhance the expression of systemically delivered genes is selectively targeted at both the tissue and cellular levels, without requiring the use of ligand- or promoter-based approaches. Analyzing HDAC-based targeting of gene expression may identify host genes that control the expression of systemically delivered genes.Gene Therapy (2006) 13, 1724–1730. doi:10.1038/sj.gt.3302825; published online 3 August 2006
- Subjects
HISTONE deacetylase; CANCER cells; CANCER patients; DNA; GENE expression; LIGANDS (Biochemistry)
- Publication
Gene Therapy, 2006, Vol 13, Issue 24, p1724
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3302825