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- Title
B-cell homeostasis requires complementary CD22 and BLyS/BR3 survival signals.
- Authors
Smith, Susan H.; Haas, Karen M.; Poe, Jonathan C.; Yanaba, Koichi; Ward, Christopher D.; Migone, Thi-Sau; Tedder, Thomas F.
- Abstract
Peripheral B-cell numbers are tightly regulated by homeostatic mechanisms that influence the transitional and mature B-cell compartments and dictate the size and clonotypic diversity of the B-cell repertoire. B-lymphocyte stimulator (BLyS, a trademark of Human Genome Sciences, Inc.) plays a key role in regulating peripheral B-cell homeostasis. CD22 also promotes peripheral B-cell survival through ligand-dependent mechanisms. The B-cell subsets affected by the absence of BLyS and CD22 signals overlap, suggesting that BLyS- and CD22-mediated survival are intertwined. To examine this, the effects of BLyS insufficiency following neutralizing BLyS mAb treatment in mice also treated with CD22 ligand-blocking mAb were examined. Combined targeting of the BLyS and CD22 survival pathways led to significantly greater clearance of recirculating bone marrow, blood, marginal zone and follicular B cells than either treatment alone. Likewise, BLyS blockade further reduced bone marrow, blood and spleen B-cell numbers in CD22−/− mice. Notably, BLyS receptor expression and downstream signaling were normal in CD22−/− B cells, suggesting that CD22 does not directly alter BLyS responsiveness. CD22 survival signals were likewise intact in the absence of BLyS, as CD22 mAb treatment depleted blood B cells from mice with impaired BLyS receptor 3 (BR3) signaling. Finally, enforced BclxL expression, which rescues BR3 impairment, did not affect B-cell depletion following CD22 mAb treatment. Thus, the current studies support a model whereby CD22 and BLyS promote the survival of overlapping B-cell subsets but contribute to their maintenance through independent and complementary signaling pathways.
- Subjects
B cells; HUMAN genome; IMMUNE system; HOMEOSTASIS; BONE marrow; ANTIGENS
- Publication
International Immunology, 2010, Vol 22, Issue 8, p681
- ISSN
0953-8178
- Publication type
Article
- DOI
10.1093/intimm/dxq055