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- Title
Development of Microsatellite Markers for Cynops ensicauda (Amphibia: Caudata) by Using Next-generation Sequencing Technology.
- Authors
MOCHIDA, KOJI; INOUE, EIJI; KURITA, KAZUKI; HAYANO, AZUSA; INOUE-MURAYAMA, MIHO
- Abstract
We isolated and characterized seven microsatellite markers including di-, tri-, and tetra-nucleotide repeats for Cynops ensicauda, which is a near-threatened species and endemic to the Central Ryukyus, Japan, using next-generation sequencing. Six out of the seven loci that showed polymorphism in Cynops ensicauda popei on Okinawajima Island were also amplified in C. e. ensicauda on Amamioshima Island. Expected heterozygosities of these markers were 0.00-0.738 (mean, 0.429) and 0.00-0.791 (mean, 0.459) in C. e. popei and C. e. ensicauda, respectively. These markers will be useful for genetic studies, such as evaluating the genetic diversity within and between populations of this species.
- Subjects
JAPAN; MICROSATELLITE repeats; BIOMARKERS; NUCLEOTIDES; ENDANGERED species
- Publication
Current Herpetology, 2012, Vol 31, Issue 2, p117
- ISSN
1345-5834
- Publication type
Article
- DOI
10.5358/hsj.31.117