We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of Excimer Laser Treatment of Zirconia Disks on the Adhesion of L929 Fibroblasts.
- Authors
Akashi, Yoshihiko; Shimoo, Yoshiaki; Hashiguchi, Hayato; Nakajima, Kei; Kokubun, Katsutoshi; Matsuzaka, Kenichi
- Abstract
The adhesion of zirconia and soft tissue is very important for the success of zirconia implants. The aim of this study was to characterize the effects of excimer laser treatment of zirconia on the adhesion of L929 fibroblasts. In this study, polished zirconia disks treated with an excimer laser were the experimental group and untreated zirconia disks were the control group. Surface roughness and contact angles of zirconia disks were measured. mRNA expression levels of integrin β1 and collagen type I α1 in L929 fibroblasts cultured on zirconia disks were measured using qRT-PCR. Cell morphology was evaluated using 3D laser microscopy and the expression of vinculin was characterized using confocal microscopy. There was no significant difference in the surface roughness of zirconia disks, but contact angles were significantly lower. mRNA expression of integrin β1 was significantly higher at 3, 6 and 24 h and of collagen type I α1 was significantly higher at 6 and 24 h. L929 fibroblasts tended to form elongated microspikes and vinculin colocalization in those microspikes. Furthermore, vinculin was strongly expressed in filopodia of L929 fibroblasts at 24 h. These results suggest that excimer laser treatment improves adhesion between zirconia disks and L929 fibroblasts.
- Subjects
EXCIMER lasers; ZIRCONIUM oxide; CELL morphology; CONTACT angle; LASER microscopy; SURFACE roughness; FIBROBLASTS
- Publication
Materials (1996-1944), 2023, Vol 16, Issue 1, p115
- ISSN
1996-1944
- Publication type
Article
- DOI
10.3390/ma16010115