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- Title
Characterization of polymorphic microsatellite markers for the green turtle ( Chelonia mydas).
- Authors
DUTTON, PETER H.; FREY, AMY
- Abstract
We describe primers and polymerase chain reaction conditions to amplify 12 microsatellite loci from the green turtle (Chelonia mydas), including one dinucleotide, four trinucleotide and seven tetranucleotide loci. The primers were tested on 78 individuals from a Pacific population nesting in the Hawaiian Islands. The primer pairs developed in this study yielded an average of 8.33 alleles per locus (range of 3–15 alleles), an average observed heterozygosity of 0.668 (range 0.309–0.910), and an average polymorphic information content of 0.647 (range 0.287–0.894).
- Subjects
GREEN turtle; POLYMERASE chain reaction; ANIMAL populations; GENETIC polymorphisms; MICROSATELLITE repeats; GENETIC markers
- Publication
Molecular Ecology Resources, 2009, Vol 9, Issue 1, p354
- ISSN
1755-098X
- Publication type
Article
- DOI
10.1111/j.1755-0998.2008.02443.x