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- Title
Effectively improved 3-dimensional structural stability of atelocollagen-gelatin sponge biomaterial by heat treatment.
- Authors
Longqiang YANG; Tadashi MIURA; Masataka KASAHARA
- Abstract
Atelocollagen-gelatin (ACG) sponge was fabricated from atelocollagen and gelatin by lyophilization without introducing toxic substances. This study aimed to investigate the effects of heat treatment on the 3-dimensional structural stability of ACG sponge biomaterial. ACG sponge samples were fabricated and heat treated at 125°C for 12 h in the vacuum. The results revealed that heat treatment did not affect porosity, pore size and mechanical compressive strength. Heat-treated ACG sponge showed decreased absorbance and peak shift of amid I (C=O) stretches, slightly higher water uptake degree and significantly decreased in vitro degradation rate. Moreover, heat-treated ACG sponge maintained good 3-dimensional surface morphology and porous microstructure throughout 7 days, while non-heat-treated ACG sponge collapsed in less than 24 h. The human mesenchymal stromal cells (hMSCs) were shown to adhere and grow well on heat-treated ACG sponges. These results indicate that heat treatment is effective and safe to stabilize 3-dimensional ACG sponge biomaterial for tissue engineering.
- Subjects
HEAT treatment; STRUCTURAL stability; POISONS; STROMAL cells; SURFACE morphology
- Publication
Dental Materials Journal, 2022, Vol 41, Issue 3, p337
- ISSN
0287-4547
- Publication type
Article
- DOI
10.4012/dmj.2021-136