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- Title
Influence of lyophilization factors and gelatin concentration on pore structures of atelocollagen/gelatin sponge biomaterial.
- Authors
Longqiang YANG; Koji TANABE; Tadashi MIURA; Masao YOSHINARI; Shinji TAKEMOTO; Seikou SHINTANI; Masataka KASAHARA
- Abstract
This study aimed to investigate influences of lyophilization factors and gelatin concentration on pore structures of ACG sponge. ACG sponges of different freezing temperatures (-30, -80 and -196oC), freezing times (1, 2 and 24 h), gelatin concentrations (0.6%AC+0.15%G, 0.6%AC+0.6%G and 0.6%AC+2.4%G), and with 500 µM fluvastatin were fabricated. Pore structures including porosity and pore size were analyzed by scanning electron microscopy and ImageJ. The cytotoxic effects of ACG sponges were evaluated in vitro. Freezing temperature did not affect porosity while high freezing temperature (-30oC) increased pore size. The high gelatin concentration group (0.6%AC+2.4%G) had decreased porosity and pore size. Freezing time and 500 µM fluvastatin did not affect pore structures. The cytotoxicity and cell proliferation assays revealed that ACG sponges had no cytotoxic effects on human mesenchymal stromal cell growth and proliferation. These results indicate that ACG sponge may be a good biomaterial scaffold for bone regeneration.
- Subjects
FREEZE-drying; GELATIN; BIOMATERIALS; FLUVASTATIN; MESENCHYMAL stem cells; SCANNING electron microscopy
- Publication
Dental Materials Journal, 2017, Vol 36, Issue 4, p429
- ISSN
0287-4547
- Publication type
Article
- DOI
10.4012/dmj.2016-242