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- Title
Molecular characterization of cotton leaf curl Multan beta-satellite occurance on chili plants in Multan, Pakistan.
- Authors
Azeem, Hajra; Ali, Amjad; Perveen, Rashida; ud din Umar, Ummad; Zakria, Muhammad; Ölmez, Fatih; Azeem, Muhammad Usman
- Abstract
The prevalence of cotton leaf curl disease (CLCuD) has significantly hampered chili (Capsicum spp.) production, presenting a formidable challenge in Pakistan. During a chili field survey in 2018, distinct symptoms, including stunted growth, yellowing, and severe leaf curling, were observed on several plants. Subsequently, a comprehensive sampling effort was undertaken, collecting a total of 39 symptomatic samples from diverse locations across Multan, Punjab. The DNA extraction from these samples was conducted at the plant virology laboratory at Bahauddin Zakariya University, Multan, marking a crucial step in the investigation of this debilitating disease and its impact on chili production in the region. Molecular analysis with PCR using Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33 primers confirmed begomovirus infection in chili plants. Positive amplification demonstrated a 71.79% infection rate, with 579 bp, 1.4 kb, and 481 bp amplicons for Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33, respectively. Sequencing identified cotton leaf curl Multan beta-satellite (MT668934) infecting the chili plant. Effectively managing these begomoviruses is crucial to curbing their multiplication and protecting vital crops like chili. Addressing the distributions of beta-satellites in agricultural fields, particularly chili, is imperative to prevent further viral spread.
- Subjects
MULTAN (Pakistan); PAKISTAN; PLANT viruses; STUNTED growth; COTTON; AGRICULTURE; VIRAL transmission; HOT peppers; GREENHOUSES
- Publication
Journal of Plant Protection Research, 2024, Vol 64, Issue 2, p139
- ISSN
1427-4345
- Publication type
Article
- DOI
10.24425/jppr.2024.150248