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- Title
Analysis of novel endosome-to-Golgi retrieval genes reveals a role for PLD3 in regulating endosomal protein sorting and amyloid precursor protein processing.
- Authors
Mukadam, Aamir S.; Breusegem, Sophia Y.; Seaman, Matthew N. J.
- Abstract
The processing of amyloid precursor protein (APP) to the neurotoxic pro-aggregatory Aβ peptide is controlled by the mechanisms that govern the trafficking and localisation of APP. We hypothesised that genes involved in endosomal protein sorting could play an important role in regulating APP processing and, therefore, analysed ~ 40 novel endosome-to-Golgi retrieval genes previously identified in a genome-wide siRNA screen. We report that phospholipase D3 (PLD3), a type II membrane protein, functions in endosomal protein sorting and plays an important role in regulating APP processing. PLD3 co-localises with APP in endosomes and loss of PLD3 function results in reduced endosomal tubules, impaired trafficking of several membrane proteins and reduced association of sortilin-like 1 with APP.
- Subjects
ENDOSOMES; AMYLOID beta-protein precursor; PEPTIDES; PHOSPHOLIPASE D; ALZHEIMER'S disease
- Publication
Cellular & Molecular Life Sciences, 2018, Vol 75, Issue 14, p2613
- ISSN
1420-682X
- Publication type
Article
- DOI
10.1007/s00018-018-2752-9