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- Title
Chondrocyte viability in press-fit cryopreserved osteochondral allografts
- Authors
Gole, Madhura D.; Poulsen, Dan; Marzo, John M.; Ko, Seung-Hee; Ziv, Israel
- Abstract
The viability of chondrocytes in press-fit glycerol-preserved osteochondral allografts was compared to that in fresh autografts, after transplantation into load-bearing and non-load-bearing sites in mature sheep stifle joints. We used macroscopic grading, tonometer pen indentation testing, histology, sulfate uptake and viability as determined by confocal-microscopy to assess cartilage condition. Despite there being no statistical differences between macroscopic appearance and tonometer testing of all grafts, confocal microscopy and histology demonstrated a positive effect of load-bearing placement on cryopreserved osteochondral allografts. Allografts transplanted into load-bearing sites demonstrated superior confocal microscopy-measured chondrocyte viability (77% ± 17%SD) than those transplanted into non-load-bearing sites (25% ± 2%). Load-bearing effect was not seen in autografts (78% ± 15%), and was comparable in adjacent cartilage (83% ± 9%). Similarly, load-bearing allografts demonstrated histological scoring closer to that of autografts and adjacent cartilage, all of which fared significantly better than non-load-bearing allografts. Load-bearing allografts had a greater amount of fibrocartilage than autografts or adjacent cartilage but less fibrocartilage than non-load-bearing allografts. Both autografts and allografts had non-significant increases in metabolism compared to adjacent cartilage as measured by sulfate-uptake. Load-bearing placement improved chondrocyte viability of glycerol cryopreserved osteochondral allograft following a press-fit implantation.
- Subjects
CARTILAGE cells; HOMOGRAFTS; JOINTS (Anatomy); TRANSPLANTATION of organs, tissues, etc.
- Publication
Journal of Orthopaedic Research, 2004, Vol 22, Issue 4, p781
- ISSN
0736-0266
- Publication type
Article
- DOI
10.1016/j.orthres.2003.11.006