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- Title
Imaging of the Inflammatory Response in Reperfusion Injury after Transient Cerebral Ischemia in Rats: Correlation of Superparamagnetic Iron Oxide-Enhanced Magnetic Resonance Imaging with Histopathology.
- Authors
Kim, J.; Kim, D. I.; Lee, S. -K.; Kim, D. J.; Lee, J. E.; Ahn, S. K.
- Abstract
Background: Acute inflammatory responses have been thought to play a central role in ischemia-reperfusion injury after acute ischemic stroke. Superparamagnetic iron oxide (SPIO) particles have been known to enable in-vivo monitoring of macrophage infiltration by magnetic resonance imaging (MRI) in the experimental ischemic rat brain. Purpose: To determine whether the accumulation of macrophages could be seen in vivo in a reperfusion animal model after focal cerebral ischemia using SPIO-enhanced MRI. Material and Methods: Thirty-four adult male rats were enrolled in this study. SPIO particles were injected into the rats at different time points after 1-hour transient occlusion of the middle cerebral artery, and three-dimensional (3D) T2*-weighted magnetic resonance (MR) images with a gradient-echo sequence were performed 24 hours later. Histochemical iron staining was compared with T2* signal abnormalities. Results: At days 3 and 4 post-reperfusion, focal areas of signal loss indicating local accumulation of SPIO particles appeared in a part of the damaged brain. Areas of signal loss corresponded to local accumulation of iron-laden macrophages in histologic sections, and SPIO-induced signal loss indicated active macrophage transmigration into the reperfused brain. Conclusion: SPIO-enhanced MRI demonstrated through in-vivo monitoring that macrophages participate in reperfusion injury at early stages of injury development. SPIO-enhanced MRI could be a useful tool to examine the inflammatory mechanisms involved in reperfusion brain injury.
- Subjects
REPERFUSION injury; MEDICAL imaging systems; MAGNETIC resonance imaging; DIAGNOSTIC imaging; CEREBRAL ischemia; RATS; DIAGNOSIS
- Publication
Acta Radiologica, 2008, Vol 49, Issue 5, p580
- ISSN
0284-1851
- Publication type
Article
- DOI
10.1080/02841850802020484