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- Title
Characterization of One Phenotype of Human Periodontal Granulation-tissue Fibroblasts.
- Authors
LARJAVA, H.; HEINO, J.; KÄHÄRI, V.-M.; KRUSIUS, T.; VUORIO, E.
- Abstract
Human granulation-tissue fibroblasts were cultured from oral chronic inflammatory lesions and compared with fibroblasts of healthy gingival connective tissue with respect to cell-surface sialoglycoproteins, and the synthesis of extracellular matrix components. Granulation-tissue fibroblasts exhibited a slower growth rate and larger size than their controls. Their cell-surface sialoglycoproteins resembled those of the control cells, except that the relative amount of glycoproteins in the 140-kd region was lower. The ratio of mRNAs for proαl(I) and proαl(III) collagen chains was decreased in granulation-tissue fibroblasts, although electrophoretic fractionation of the proteins did not reveal consistent differences in type I/type III collagen ratio. Granulation-tissue fibroblasts secreted into the culture medium a dermatan sulfate proteoglycan with a lower molecular weight. After digestion with chondroitinase ABC, the molecular weight of the core protein appeared to be identical with that of the control fibroblasts, suggesting a difference in the glycosylation of the core protein. These results support the theory that granulation-tissue fibroblasts represent a distinct phenotype of fibroblastic cells.
- Subjects
GRANULATION tissue; FIBROBLASTS; PHENOTYPES; EXTRACELLULAR matrix; MESSENGER RNA; COLLAGEN
- Publication
Journal of Dental Research, 1989, Vol 68, Issue 1, p20
- ISSN
0022-0345
- Publication type
Article
- DOI
10.1177/00220345890680010301